中科院细胞与分子免疫学课程课件Chapter2Theremaybe107ormoredifferentTandBlymphocyteclonesinoneindividual,eachcloneproduceonespecificantigenreceptor.Duringthepre-Bcellorpre-Tcellstage,animmatureformofantigenreceptorisformedwhichtotransducesignalstoinducefurthermaturationMHCmoleculesinthymusareimportant.SinglepositiveTCRrecognizetheMHCI-peptidescomplex,theCD8interactswiththemolecules,theTcellwilldeveloptoCD8+Tcell.EndonucleasecleavessinglestrandatrandomsitesinVandDsegmentRecombinationinPro-Bcell:Thestimulicomefromcellsincluding:TCGACGTTATATTCRa,dchain:chromosome14SelectionofthematurerepertoriesSynthesizelow-affinityIgMinresponsetomanybacteriaFromthedouble-negativetothedouble-positivestage.BlotwithaVregionprobe4、B细胞表面受体的重排：456789101112131415161718192021222324252627282930313233343536373839404142434445464748495051ThematurationofBandTlymphocytesconsistsof:Hoststrainandtreatment-comparethepatternofbandsStepsofIggenerecombinationEarlymaturation:LymphocyteMaturationandExpressionofAntigenReceptorGenesTCGACGTTATATRecombinationinPro-Bcell:在胚系基因组中包含了免疫基因库，可对外界的众多抗原发生应答，并能遗传。1GenerationofthepalindromicsequenceSomanyspecificities–sofewgeneschromosome14:HchainlocusThepre-Tcellreceptorwasformed.RAG-1andRAG-2arefirstexpressed.Whataretheproblemswiththistheory?V:likeIgVgene535455565758596061626364656667686970717273747576777879808182谢谢观看！感谢观看