2008年3月湖北师范学院生命科学学院生物化学实验指导1目录前言···························································································································2生物化学实验守则········································································································3实验一实验基本知识及溶液的配制··········································································5实验二蛋白质及氨基酸的呈色反应··········································································9实验三蛋白质的等电点测定和沉淀反应·································································13实验四蛋白质的定量测定方法················································································17实验五离子交换柱层析法分离氨基酸·····································································25实验六凝胶层析法和SDS聚丙烯酰胺凝胶电泳法—蛋白质的分子量测定··········27实验七酶的特性········································································································34实验八底物浓度对酶促反应速度的影响·································································38实验九酪氨酸酶的提取及其催化活性研究·····························································40实验十核糖核酸的提取与组分鉴定及核酸含量的测定·········································44实验十一果蔬维生素C含量测定及其分析······························································47实验十二小麦萌发前后淀粉酶活力的比较·····························································50实验十三脂肪酸的β－氧化······················································································53实验十四氨基移换反应（一）——血液中转氨酶活力的测定·····························55实验十五氨基移换反应（二）――谷丙转氨酶活性的鉴定·································57实验十六发酵过程中无机磷的利用········································································59参考文献······················································································································62湖北师范学院生命科学学院生物化学实验指导2前言近几年来，随着生物化学学科的迅猛发展，教学内容更新较快。生化实验被作为主干基础课列入了高等院校生命科学各专业的教学计划中，并且实验课单独作为一门课程开出。为了提高教学水平，反映出本学科更具科学性、先进性和实用性的实验内容，根据教学大纲的要求和课程设置，同时根据本校教学实验条件，在参阅中外相关文献，学习兄弟院校的教学经验和资料的基础上，编写了本实验讲义。本书为本校生