长春工业大学学士学位论文摘要中国林蛙（RanachensinensisDavid）又名蛤什蟆，属两栖纲、无尾目、蛙科、蛙属，是集药用、食补、美容功能于一体的珍稀两栖类动物，具有很高的应用价值和开发潜力。本文以东北林蛙皮为实验材料，以多糖的提取率为考察指标，将林蛙皮粉碎，过筛，活性炭脱腥，比较热水提取法、碱提取法、酶提取法三种不同提取方法的提取效果，并用苯酚-硫酸法测定多糖含量。实验结果表明，酶法提取效果最佳，其次是热水提取法和碱提取法。采用正交试验法对酶法提取多糖的工艺进行优化，得出其最佳提取工艺条件为：酶用量2%，胃蛋白酶与胰蛋白酶比2:1，提取温度50℃，提取时间7h，多糖提取率为0.91%。关键词：林蛙皮；多糖；酶法；正交试验AbstractChineseforestfrog(RanachensinensisDavid)akaHashima,belongingtotheAmphibia,Anura,frog,Rana,isasetofmedical,food,cosmeticfunctionsinoneoftherareamphibiananimal,hasveryhighvalueforapplicationandpromisingfuture.TheskinofRanadybowskiiisusedasexperimentalmaterial,withtheextractionrateofpolysaccharideasanindex.Crushingtheforestfrogskin,sievingit,activatedcarbondeodorization,comparedwithhotwaterextraction,alkaliextraction,enzymeextractionofthreedifferentextractionmethods,andbyphenolsulfuricacidmethodforthedeterminationofpolysaccharidecontent.Theexperimentalshowsthatenzymaticextractionhasthebesteffect,,followedbyhotwaterextractionandalkaliextraction.Usingtheorthogonaltestmethodonenzymeextractionpolysaccharidetechnologyoptimization,theoptimumextractionconditionsforenzymedosage:2%enzyme,pepsinandtrypsinthan2:1,extractiontemperatureis50℃,extractiontimeis7h,polysaccharidesextractionrateis0.91%.Keywords:forestfrogskin;polysaccharide;enzymemethod;orthogonaltest目录TOC\o"1-3"\h\uHYPERLINK\l_Toc4507摘要PAGEREF_Toc45071HYPERLINK\l_Toc24660AbstractPAGEREF_Toc246601HYPERLINK\l_Toc9999目录PAGEREF_Toc99992HYPERLINK\l_Toc17048第一章前言PAGEREF_Toc170484HYPERLINK\l_Toc18303第二章文献综述PAGEREF_Toc183035HYPERLINK\l_Toc323082.1中国林蛙PAGEREF_Toc323085HYPERLINK\l_Toc261212.1.1中国林蛙的特征PAGEREF_Toc261215HYPERLINK\l_Toc138522.1.2林蛙的功能作用PAGEREF_Toc138526HYPERLINK\l_Toc278652.1.3林蛙的国内外研究现状PAGEREF_Toc278657HYPERLINK\l_Toc232212.2林蛙皮PAGEREF_Toc232217HYPERLINK\l_Toc15652.2.1林蛙皮的功能PAGEREF_Toc15657HYPERLINK\l_Toc254362.2.2林蛙皮研究现状和应用前景PAGEREF_Toc2543610HYPERLINK\l_Toc127712.3多糖PAGEREF_To