Y-27632对家兔心肌缺血/再灌注损伤的有益作用推荐信息：医学论文发表——创新医学网http://www.yixue360.com/作者：梁洪英，郑惠珍，吴纯英，陈智文，吴希春作者单位：524023广东湛江，广东医学院生理科学实验室(△生理研究室，通讯作者，▲第一临床学院)【摘要】目的研究Rho激酶抑制剂Y-27632对家兔心肌缺血/再灌注损伤(IRI)是否有心脏保护作用。方法健康家兔18只，体重2.0～2.5kg，随机分为3组：IRI组、Y-27632(Y)组和假手术(C)组。用结扎左冠状动脉前降支60min后剪断结扎线恢复血流30min的方法建立在体心肌IRI模型，Y组于再灌注前5min，经耳缘静脉注射Y-276320.35mg/kg。检测左心室收缩功能，血浆肌酸激酶(CK)、乳酸脱氢酶(LDH)及心肌缺血、梗死范围。结果IRI组缺血/再灌注导致左心室舒缩功能受损，左心室收缩峰压(LVSP)、左心室内压最大上升速率(+dp/dtmax)下降，左心室舒张末期压力(LVEDP)升高，CK、LDH的释放增加，心肌梗死范围为(5.3±0.7)%;Y组在再灌注早期LVSP、±dp/dtmax下降的幅度和LVEDP升高的幅度、CK和LDH的释放，均明显低于IRI组，组间比较P均<0.05。心肌缺血、梗死范围也明显较IRI组小(缺血：40.5%υs51.3%，P<0.05;梗死：1.7%υs5.3%，P<0.01)。结论Y-27632对家兔心肌IRI有部分心脏保护作用。【关键词】缺血/再灌注损伤;Rho激酶;心脏;家兔EffectsofY-27632onmyocardialischemia-reperfusioninjuryinrabbitsLIANGHong-ying，ZHENGHui-zhen，WUChun-ying，etal.DepartmentofPhysiology，GuangdongMedicalCollege，Zhanjiang524023，China[Abstract]ObjectiveToobservetheeffectsofaRhokinaseinhibitor，Y-27632，onischemia-reperfusioninjury(IRI)inrabbitheartinvivo.MethodsEighteenrabbitswererandomlydividedintothreegroups:ischemiareperfusion(IRI)group，ischemiareperfusionplusY-27632(Y)groupandshamoperation(C)group.Theanimalsweresubjectedto60minofleftanteriordescendingcoronaryarteryocclusionand30minreperfusioninIRIgroup.TheyweretreatedwithY-27632at350μg/kgbyintravenousinjection5minbeforereperfusioninYgroup.ResultsIschemia-reperfusionledtomyocardialinfarction，theleakageofcreatinekinase(CK)andlactatedehydrogenase(LDH)andcardiofunctiondecrease.Y-27632reducedischemiareperfusion-inducedmyocardialinfarction(1.7%υs5.3%，P<0.01，comparedwithIRgroup).Italsoreducedischemiareperfusion-inducedtheleakageofCKandLDH(28.9%υs34.9%and45.7%υs79%，respectivelyP<0.05).Moreover，Y-27632enhancedpost-reperfusionmyocardialsystalticfunction，leftventricularsystolicpressure(LVSP)，themaximalrateofleftventricularpressurerise(+dp/dtmax)andleftventricularend-diastolicpressure(LVEDP)weresignificantlyimprovedinY-27632groupascomparedwithIRIgroup.ConclusionTheseresultssuggestthatY-27632exertspartiallycardioprote