攀枝花学院本科毕业设计（论文）摘要攀枝花学院本科毕业设计（论文）磷在锰的胁迫下对蚕豆根尖有丝分裂的影响院（系）：生物与化学工程学院年级专业：2009级生物工程班二〇一三年六月攀枝花学院本科毕业设计（论文）摘要PAGE\*MERGEFORMATII摘要本课题为探究磷在锰的胁迫下对蚕豆根尖有丝分裂的影响。分别由氯化锰和磷酸二氢钾提供锰素与磷素。用一定的锰浓度与不同浓度的磷复合处理蚕豆根尖，然后镜检观察蚕豆根尖细胞染色体畸变情况。结果表明当用2mmol/L的锰分别与13mmol/L、1.3mmol/L、0.13mmol/L、0.013mmol/L、0.0013mmol/L的磷复合处理根尖并通过三次平行实验后，在该实验条件下蚕豆根尖细胞平均有丝分裂指数呈现先增后减的趋势，在磷浓度为0.13mmol/L时最高达42.6‰；而平均染色体畸变率呈现先减后增的趋势，磷浓度为0.13mmol/L时最低至33.7％。说明在该实验条件下，有丝分裂指数和染色体畸变率与该复合培养液有明显的剂量——效应关系。关键词：蚕豆、细胞分裂指数、染色体畸变率、KH2PO4、MnCl2攀枝花学院本科毕业设计（论文）ABSTRACTPAGE\*MERGEFORMATIIIABSTRACTThispaperexploresthephosphorusmitogenicinmanganesestresseffectsonmitosisofViciafabaroottip.ByMn2Cl﹒4H2OandKH2PO4withmanganeseandphosphorus.ManganeseconcentrationwithdifferentconcentrationsofphosphoruscompoundtreatmentofViciafabaroottip,andobservingthechromosomeaberrationinroottipcellsofViciafababymicroscope.Theresultshowsthat,whenusingthe2mmol/Lmanganesephosphorusconcentrationcompoundtreatmentwith13mmol/L,1.3mmol/L,0.13mmol/Lroot,0.013mmol/L,0.0013mmol/L,obtainedbythreeparallelexperiment:Undertheexperimentalconditions，theaveragemitoticindexofViciafabaroottipcellincreasedfirstlyandthendecreased,thephosphorusconcentrationfor0.13mmol/L42.6‰washighest;Whiletheaveragechromosomeaberrationrateshowedthetrendofdecreasedfirstlyandthenincreased,phosphorusconcentrationwas0.13mmol/Lforaminimumof33.7%.Theresultshowsthat:undertheexperimentalconditions,thereisrate-effectrelationshipbetweenmitoticindexandchromosomeaberrationandthecompositemediumsignificantly.Keywords:Viciafaba,mitoticindex,therateofchromosomeaberration,KH2PO4,MnCl2攀枝花学院本科毕业设计（论文）目录目录TOC\o"1-3"\h\z\uHYPERLINK\l"_Toc357425254"摘要PAGEREF_Toc357425254\hIHYPERLINK\l"_Toc357425255"ABSTRACTPAGEREF_Toc357425255\hIIHYPERLINK\l"_Toc357425256"1绪论PAGEREF_Toc357425256\h1HYPERLINK\l"_Toc357425257"1.1课题背景PAGEREF_Toc357425257\h1HYPERLINK\l"_Toc357425258"1.1.1引言PAGEREF_Toc357425258\h1HYPERLIN