上海海洋大学2012届毕业设计（论文）中草药抗氧化活性研究—忍冬藤活性研究毕业设计（论文）题目：中草药抗氧化活性研究——忍冬藤抗氧化活性学院：专业：班级：姓名学号：指导教师目录中英文摘要···················································1绪论·························································21忍冬藤提取工艺··············································21.1所需仪器···············································21.2提取方法···············································22忍冬藤抗氧化活性研究方法···································32.1DPPH法··················································32.1.1DPPH法实验原理·····································32.1.2DPPH法实验过程·····································32.1.3DPPH法实验分析与结果·······························32.2福林酚法················································52.2.1福林酚法实验原理···································52.2.2福林酚实验过程·····································52.2.3福林酚实验分析与结果·······························62.3ABTS法·················································72.3.1ABTSS实验原理······································72.3.2ABTS实验过程·······································72.3.3ABTS实验分析与结果·································82.4铁离子螯合能力法·······································92.4.1铁离子螯合实验原理·······························92.4.2铁离子螯合实验过程·······························92.4.3铁离子螯合实验分析与结果·························102.5还原力测定法···········································102.5.1还原力实验原理···································102.5.2还原力实验过程···································112.5.3还原力实验分析与结果·····························123总结························································13致谢··························································14参考文献······················································15第PAGE\*MERGEFORMAT15页中草药抗氧化活性研究——忍冬藤抗氧化活性摘要中草药是天然的抗氧化活性物质的重要来源，因其种类繁多，如菊花，甘草，忍冬藤，金银花，决明子，等中草药抗氧化成分复杂，因此活性差异较大。用于开发体内自由基清除药物，保健品，和食品的抗氧化剂。中草药中的抗氧化活性物质有黄酮，多酚，生物碱，多糖，皂苷等等，都是非常好的天然抗氧化活性成分。本文以忍冬藤为例。其成分有皂苷，绿原酸，黄酮，多糖，都具有清除自由基能力和抗菌能力。忍冬藤能采用多种方法分析其抗氧化活性。DPPH方法是目前最常用的检测清除自由基能力被广泛应用于实验室中。福林酚法用于检测样品中的总酚含量，是研究抗氧化活性的因素之一。ABTS法原理与DPPH相似，