山茶油脂肪酸甲酯化条件研究（完整版）实用资料(可以直接使用，可编辑完整版实用资料，欢迎下载）山茶油脂肪酸甲酯化条件研究唐芳1,李小元1,吴卫国1,刘军鸽2(1.湖南农业大学食品科技学院,食品科学与生物技术湖南省重点试验室,湖南长沙410128;2.湖南农业大学分析测试中心,湖南长沙410128摘要:油脂甲酯法是应用气相色谱法测定油脂脂肪酸组成一个必不可少预处理工序。该文报道对比研究KOH―甲醇溶液法、H2SO4―甲醇溶液法对山茶油进行甲酯化效果,着重探讨振荡温度、振荡时间、催化剂用量等因素对山茶油甲酯化效果影响规律,通过气相色谱法测定油酸峰面积及油酸甲酯转化率为考核指标,优化KOH―甲醇溶液法和H2SO4―甲醇溶液法甲酯化条件,并提出酸碱结合甲酯化新方法,研究结果表明,采用酸碱结合法对山茶油进行甲酯化效果优于KOH―甲醇溶液法和H2SO4―甲醇溶液法,对应用气相色谱法测定油脂脂肪酸组成具有实际指导意义。关键词:山茶油;甲酯化;KOH―甲醇溶液法;H2SO4―甲醇溶液法;酸碱结合法StudyonmethylesterificationconditionsoffattyacidincamelliaoilTANGFang1,LIXiao-yuan1,WUWei-guo1,LIUJun-ge2(1.CollegeofFoodScience&TechnologyofHunanAgriculturalUniversity,FoodScience&BiotechnologyofKeyLaboratoryofHunanProvince,Changsha410128,China;2.CenterofAnalyticTestingofHunanAgriculturalUniversity,Changsha410128,ChinaAbstract:Themethylesterificationmethodofoilswasanessentialpretreatmentprocessforthedeterminationoffattyacidcompositioninoilsbygaschromatography.ThepaperstudiedtheeffectsofmethylesterificationincamelliaoilthroughthecomparationofKOH–methanolsolutionmethod,H2SO4–methanolsolutionmethod;andexploredtherulethattheoscillationsoftemperatureandtime,thecontentsofcatalystandotherfactorswouldimpactedontheeffectofmethylesterificationincamelliaoil.Withpeakareaofoleicacidgaschromatographydeterminedandtheefficiencyofoleicacidmethylesterasanindicatorsfortheassessment,itoptimizedtheconditionsofmethylesterificationofKOH–methanolsolutionmethod,andH2SO4–methanolsolutionmethod,andmadeanewmethodofmethylesterificationofacid–alkalicombination.Theresultsonstudyindicatedthatacid–alkalicombinationmethodfortheeffectofmethylesterificationincamelliaoilwassuperiortoKOH–methanolsolutionmethodandH2SO4–methanolsolutionmethodforthat,andthatithadasignificanceofthepracticalguidetodeterminethecompositionoffattyacidinoleinbygaschromatography.Keywords:camelliaoil;methylesterification;KOH–methanolsolutionmethod;H2SO4–methanolsolutionmethod;acid–alkalicombinationmethod中图分类号:O657.7+1文献标识码:A文章编号:1008―9578(202108―0036―04收稿日期:2021–07–03作者简介:唐芳(1985~