毕业论文（设计）题目：葡萄WRKY13参与对植株生长调控及对ABA的应答PAGE\*MERGEFORMAT4目录中文摘要……………………………………………………………………………………….1Abstract…………………………………………………...…………………………………....2引言………………………………………………………………………………………….....41材料与方法.............................................................................................................................61.1实验材料..............................................................................................................................61.1.1菌株及载体........................................................................................................................61.1.2常用酶、化学药品、试剂盒...........................................................................................61.1.3实验仪器...........................................................................................................................61.2实验方法..............................................................................................................................61.2.1材料培养...........................................................................................................................61.2.2材料处理...........................................................................................................................61.2.3培养基的配置...................................................................................................................71.2.4重组质粒的鉴定...............................................................................................................71.2.5根癌农杆菌（GV3101）感受态细胞的制备及转化.........................................................71.2.6花芽浸蘸法转化拟南芥及阳性植株的筛选...................................................................81.2.7气孔开度测定...................................................................................................................81.2.8实时定量PCR...................................................................................................................82结果与分析...............................................