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III.ChromosomereplicationiscoordinatedwithcelldivisionIII.ChromosomereplicationiscoordinatedwithcelldivisionIII.ChromosomereplicationiscoordinatedwithcelldivisionIII.ChromosomereplicationiscoordinatedwithcelldivisionIV.SomemethodsforDNAstudyNucleicacidshybridizebybasepairingDNAMeltingDenaturedsinglestrandsofDNAcanrenaturetogivetheduplexform.FilterhybrydizationCalculationofTmforaDNAmoleculeMeasurementofnucleicacidconcentrationRestrictionenzymesRestrictionandmodification(限制與修飾)RestrictionenzymesRestrictionfragmentendsSeparationofDNAfragmentsbygelelectrophoresisElectrophoresiscanseparateDNAfragmentsfromoneanother（電泳可將DNA片段彼此分開）Horizontalapparatusfor(agarose)gelelectrophoresismovementofDNAinelectricalfieldGelelectrophoresisforDNAfragmentsPulsed-FieldGelElectrophoresis(PFGE)SouthernblotanalysisPCRreaction(聚合酵素鏈鎖反應)PCRreaction(聚合酵素鏈鎖反應)TheDeterminingbasesequenceofDNAfragments（定序列）Maxam&GilbertSequencingStructureofanα-35S-deoxynucleotidetriphosphateDideoxynucleosidetriphosphates(ddNTP)DNAsequencingwithddNTPsaschainterminatorsProfileofanautomatedsequencingLabellingtheProbes3‘-end-labelling
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