GenesinaBottleWorkshopTimelineRelevanceofDNAIsolationDNAStructure双螺旋结构碱基对=阶梯排列顺序=信息“文本”MethodsofDNAExtraction1.GenomicDNA2.Non-genomicDNAMethodsofDNAExtraction1.GenomicDNA2.Non-genomicDNAWhataretheStructuresoftheCell?MethodsofGenomicDNAExtraction生物医学科学研究技能IGenesinaBottleKitCellCollectionGentlychewingtheinsideofthemouthcombinedwithawatermouthwashisusedtodislodgeepithelialcellsliningthemouthAmplecellcollectioniscriticalforsuccess.2.LysisBufferWhatisLysisBuffer?•100mMTris-HCI,pH8.5•0.2%SDS•5mMEDTA•200mMNaClTrisbuffertomaintainthepHofthesolutionatalevelwhereDNAisstableSDStobreakopenthecellandnuclearmembranes,allowingtheDNAtobereleasedintothesolution(SDSalsodenaturesandunfoldsproteins,makingthemmoresusceptibletoproteasecleavage).EDTAtoinhibitDnase3.WhyAddProtease?4.AddingSalt5.AddingIceColdAlcohol?GenesinaBottleHowLongDoestheDNAintheNecklaceLast?